EsturgeonV1, France, Analysis, bibRecord, 000169

Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability

Identifieur interne : 000169 ( France/Analysis ); précédent : 000168; suivant : 000170

Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability

Auteurs : Stefano Peruzzi [France] ; Beatrice Chatain [France]

Source :

Aquaculture [ 0044-8486 ] ; 2000.

RBID : ISTEX:137D45329C8625CEAF8D45F18F986669F4B64709

Abstract

The optimal conditions for the retention of the second polar body in sea bass eggs were investigated by altering the timing, intensity and duration of application of pressure and cold shocks. Treatment optima for cold shocks were 0–1°C for 15–20 min at 5 min after fertilisation (a.f.) and 8500 psi for 2 min at 6 min a.f. for pressure shocks. Meiogenesis was obtained by fertilising eggs with UV-irradiated homologous sperm (32,000 erg mm−2) and pressure or cold shocking eggs as above. 100% triploidy was induced following definition of liable periods for the disruption of the meiotic spindle obtained in gynogenesis. Ploidy investigations were performed on experimental groups by flow-cytometry. Verification of uniparental transmission in meiogens was carried out by microsatellite marker loci analysis. This work highlights the degree of variation in individual responses of selected broodstock to these agents. Finally, some preliminary results on heterologous fertilisation in sea bass with potential applications for gynogenetic studies are also provided.

Url:

https://api.istex.fr/document/137D45329C8625CEAF8D45F18F986669F4B64709/fulltext/pdf

DOI: 10.1016/S0044-8486(00)00355-0

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 000C63
to stream Istex, to step Curation: 000C61
to stream Istex, to step Checkpoint: 000C50
to stream Main, to step Merge: 001601
to stream Main, to step Curation: 001524
to stream Main, to step Exploration: 001524
to stream France, to step Extraction: 000169

Links to Exploration step

ISTEX:137D45329C8625CEAF8D45F18F986669F4B64709

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability</title>
<author><name sortKey="Peruzzi, Stefano" sort="Peruzzi, Stefano" uniqKey="Peruzzi S" first="Stefano" last="Peruzzi">Stefano Peruzzi</name>
</author>
<author><name sortKey="Chatain, Beatrice" sort="Chatain, Beatrice" uniqKey="Chatain B" first="Beatrice" last="Chatain">Beatrice Chatain</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:137D45329C8625CEAF8D45F18F986669F4B64709</idno>
<date when="2000" year="2000">2000</date>
<idno type="doi">10.1016/S0044-8486(00)00355-0</idno>
<idno type="url">https://api.istex.fr/document/137D45329C8625CEAF8D45F18F986669F4B64709/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000C63</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000C63</idno>
<idno type="wicri:Area/Istex/Curation">000C61</idno>
<idno type="wicri:Area/Istex/Checkpoint">000C50</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000C50</idno>
<idno type="wicri:doubleKey">0044-8486:2000:Peruzzi S:pressure:and:cold</idno>
<idno type="wicri:Area/Main/Merge">001601</idno>
<idno type="wicri:Area/Main/Curation">001524</idno>
<idno type="wicri:Area/Main/Exploration">001524</idno>
<idno type="wicri:Area/France/Extraction">000169</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability</title>
<author><name sortKey="Peruzzi, Stefano" sort="Peruzzi, Stefano" uniqKey="Peruzzi S" first="Stefano" last="Peruzzi">Stefano Peruzzi</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>CEFREM, Centre de Formation et de Recherche sur l'Environnement Marin, CNRS UMR 5110, Université de Perpignan, 52 avenue de Villeneuve, 66860 Perpignan</wicri:regionArea>
<placeName><region type="region" nuts="2">Occitanie (région administrative)</region>
<region type="old region" nuts="2">Languedoc-Roussillon</region>
<settlement type="city">Perpignan</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chatain, Beatrice" sort="Chatain, Beatrice" uniqKey="Chatain B" first="Beatrice" last="Chatain">Beatrice Chatain</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Station Expérimentale d'Acquaculture IFREMER, chemin de Maguelone, 34250 Palavas-les-Flots</wicri:regionArea>
<placeName><region type="region" nuts="2">Occitanie (région administrative)</region>
<region type="old region" nuts="2">Languedoc-Roussillon</region>
<settlement type="city">Palavas-les-Flots</settlement>
</placeName>
</affiliation>
<affiliation></affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">France</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Aquaculture</title>
<title level="j" type="abbrev">AQUA</title>
<idno type="ISSN">0044-8486</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="2000">2000</date>
<biblScope unit="volume">189</biblScope>
<biblScope unit="issue">1–2</biblScope>
<biblScope unit="page" from="23">23</biblScope>
<biblScope unit="page" to="37">37</biblScope>
</imprint>
<idno type="ISSN">0044-8486</idno>
</series>
<idno type="istex">137D45329C8625CEAF8D45F18F986669F4B64709</idno>
<idno type="DOI">10.1016/S0044-8486(00)00355-0</idno>
<idno type="PII">S0044-8486(00)00355-0</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0044-8486</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The optimal conditions for the retention of the second polar body in sea bass eggs were investigated by altering the timing, intensity and duration of application of pressure and cold shocks. Treatment optima for cold shocks were 0–1°C for 15–20 min at 5 min after fertilisation (a.f.) and 8500 psi for 2 min at 6 min a.f. for pressure shocks. Meiogenesis was obtained by fertilising eggs with UV-irradiated homologous sperm (32,000 erg mm−2) and pressure or cold shocking eggs as above. 100% triploidy was induced following definition of liable periods for the disruption of the meiotic spindle obtained in gynogenesis. Ploidy investigations were performed on experimental groups by flow-cytometry. Verification of uniparental transmission in meiogens was carried out by microsatellite marker loci analysis. This work highlights the degree of variation in individual responses of selected broodstock to these agents. Finally, some preliminary results on heterologous fertilisation in sea bass with potential applications for gynogenetic studies are also provided.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Languedoc-Roussillon</li>
<li>Occitanie (région administrative)</li>
</region>
<settlement><li>Palavas-les-Flots</li>
<li>Perpignan</li>
</settlement>
</list>
<tree><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Peruzzi, Stefano" sort="Peruzzi, Stefano" uniqKey="Peruzzi S" first="Stefano" last="Peruzzi">Stefano Peruzzi</name>
</region>
<name sortKey="Chatain, Beatrice" sort="Chatain, Beatrice" uniqKey="Chatain B" first="Beatrice" last="Chatain">Beatrice Chatain</name>
<name sortKey="Chatain, Beatrice" sort="Chatain, Beatrice" uniqKey="Chatain B" first="Beatrice" last="Chatain">Beatrice Chatain</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/France/Analysis

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000169 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000169 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Eau
   |area=    EsturgeonV1
   |flux=    France
   |étape=   Analysis
   |type=    RBID
   |clé=     ISTEX:137D45329C8625CEAF8D45F18F986669F4B64709
   |texte=   Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability
}}

This area was generated with Dilib version V0.6.27.
Data generation: Sat Mar 25 15:37:54 2017. Site generation: Tue Feb 13 14:18:49 2024

	Serveur d'exploration sur l'esturgeon
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'esturgeon

Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability

Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri